![]() We previously reported that, in vitro, the lab-adapted HIV-1 NL4-3 strain can acquire resistance to the integrase inhibitor dolutegravir (DTG) by acquiring mutations in the envelope glycoprotein (Env) that enhance viral cell-cell transmission. Download FIG S4, TIF file, 0.7 MB.ĭespite the effectiveness of antiretroviral (ARV) therapy, virological failure can occur in some HIV-1-infected patients in the absence of mutations in drug target genes. Data from at least three independent experiments are shown as means ± SEs. ![]() ![]() Comparison of NL4-3 and NL(AD8) sCD4-induced (E) and time-dependent (F) gp120 shedding. ![]() Quantification of sCD4-induced (C) and time-dependent (D) gp120 shedding from three independent experiments, calculated as the ratios of virion-associated gp120/p24 and shown as means ± SEs. Incubated viruses were subsequently purified through 20% sucrose cushions, and viral proteins were detected by Western blotting. (B) Viruses were incubated at 37☌ for the indicated times. (A) Concentrated viruses were incubated with the indicated concentrations of sCD4 at 37☌ for 2 h. Analysis of sCD4-induced and time-dependent gp120 shedding of NL(AD8) Env mutants and comparison of NL4-3 versus NL(AD8) gp120 shedding.
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